Endogenous Metabolism of Azotobacter agilis

Induced biochemical mutants to Azotobacter agilis.

Endogenous encystment of Azotobacter vinelandii.

When young cells of Azotobacter vinelandii are impinged on membrane filters, washed free of carbon substrate, and placed on a mineral salts basal medium, the culture will proceed to encyst although at a slower rate than if n-butanol were supplied as a substrate. The endogenous cysts are depleted in polybeta-hydroxybutyrate and have a narrower intine but show an increased resistance to desiccati...

Metabolic Studies of Azotobacter Agilis by the Use of a Mutant Deficient in Pyruvic Oxidase*

An account has been given elsewhere of the successful isolation of physiological x-ray mutants of Azotobacter agilis with genetic blocks in the substrate oxidation mechanisms (1). The present paper deals in greater detail with a mutant strain, designated A13, that was isolated as a variant unable to grow on glucose, and gives the methods used to characterize the particular deficiency responsibl...

The effect of alkylresorcinol on lipid metabolism in Azotobacter chroococcum.

We studied the effect of exogenous alkylresorcinols on the lipid metabolism of Azotobacter chroococcum. We observed that when 5-n-pentadecylresorcinol was present in the growth medium, the more endogenous alkylresorcinols were synthesized. Concurrently, a drop in the amount of phospholipids was observed. These changes were associated with increasing numbers of dormant cysts, while the number of...

Metabolism of ribose-5-phosphate by Azotobacter vinelandii.

Because ribose-5-phosphate (R-5-P) and sedoheptulose-7-phosphate (S-7-P) have been found free in cells of the axotobacter, the mechanism of synthesis of these compounds was sought. Previously, Horecker (1) and Wood and Schwerdt (2) demonstrated in yeast and Pseudomonas Jluorestens that S-7-P was produced from R-5-P by an enzyme called transketolase. The R-5-P was first converted by an isomerase...